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. 2002 Aug;8(4):724-7.
doi: 10.3748/wjg.v8.i4.724.

Hyposmotic membrane stretch potentiated muscarinic receptor agonist-induced depolarization of membrane potential in guinea-pig gastric myocytes

Lin Li  1 , Nan-Ge Jin, Lin Piao, Ming-Yu Hong, Zheng-Yuan Jin, Ying Li, Wen-Xie Xu
Affiliations

Hyposmotic membrane stretch potentiated muscarinic receptor agonist-induced depolarization of membrane potential in guinea-pig gastric myocytes

Lin Li et al. World J Gastroenterol. 2002 Aug.

Abstract

Aim: To investigate the relationship between hyposmotic membrane stretch and muscarinic receptor agonist-induced depolarization of membrane potential in antral gastric circular myocytes of guinea-pig.

Methods: Using whole cell patch-clamp technique recorded membrane potential and current in single gastric myocytes isolated by collagenase.

Results: Hyposmotic membrane stretch hyperpolarized membrane potential from -60.0mV+/-1.0mV to -67.9mV+/-1.0mV. TEA (10 mmol/L), a nonselective potassium channel blocker significantly inhibited hyposmotic membrane stretch-induced hyperpolarization. After KCl in the pipette and NaCl in the external solution were replaced by CsCl to block the potassium current, hyposmotic membrane stretch depolarized the membrane potential from -60.0 mV+/-1.0mV to -44.8 mV+/-2.3mV (P<0.05), and atropine (1 micromol/L) inhibited the depolarization of the membrane potential. Muscarinic receptor agonist Carbachol depolarized membrane potential from -60.0mV+/-1.0mV to -50.3 mV+/-0.3mV (P<0.05) and hyposmotic membrane stretch potentiated the depolarization. Carbachol induced muscarinic current (I( cch )) was greatly increased by hyposmotic membrane stretch.

Conclusion: Hyposmotic membrane stretch potentiated muscarinic receptor agonist-induced depolarization of membrane potential, which is related to hyposmotic membrane stretch-induced increase of muscarinic current.

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Figures

Figure 1
Figure 1
Effect of hyposmotic membrane stretch on membrane potential (aP < 0.05 vs control).
Figure 2
Figure 2
Effect of TEA on membrane potential caused by hyposmotic membrane stretch (aP < 0.05 vs control).
Figure 3
Figure 3
Hyposmotic membrane stretch induced depolarization of membrane potential in the solutionn including CsCl (aP < 0.05 vs control).
Figure 4
Figure 4
Hyposmotic membrane stretch increased the depolarization of membrane potential induced by CCh in the solutionn including CsCl (aP < 0.05 vs control).
Figure 5
Figure 5
The effect of Atropine on membrane potential hyperpolarized by hyposmotic membrane stretch in the solution including CsCl (aP < 0.05 vs control).
Figure 6
Figure 6
Hyposmotic membrane stretch increased the amplitude of the current induced by Carbachol (aP < 0.05 vs control).
See this image and copyright information in PMC

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