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. 2002 Aug 20;99(17):11482-6.
doi: 10.1073/pnas.132384699. Epub 2002 Aug 12.

Loss of stearoyl-CoA desaturase-1 function protects mice against adiposity

James M Ntambi  1 , Makoto Miyazaki, Jonathan P Stoehr, Hong Lan, Christina M Kendziorski, Brian S Yandell, Yang Song, Paul Cohen, Jeffrey M Friedman, Alan D Attie
Affiliations

Loss of stearoyl-CoA desaturase-1 function protects mice against adiposity

James M Ntambi et al. Proc Natl Acad Sci U S A. .

Abstract

Stearoyl-CoA desaturase (SCD) is a central lipogenic enzyme catalyzing the synthesis of monounsaturated fatty acids, mainly oleate (C18:1) and palmitoleate (C16:1), which are components of membrane phospholipids, triglycerides, wax esters, and cholesterol esters. Several SCD isoforms (SCD1-3) exist in the mouse. Here we show that mice with a targeted disruption of the SCD1 isoform have reduced body adiposity, increased insulin sensitivity, and are resistant to diet-induced weight gain. The protection from obesity involves increased energy expenditure and increased oxygen consumption. Compared with the wild-type mice the SCD1-/- mice have increased levels of plasma ketone bodies but reduced levels of plasma insulin and leptin. In the SCD1-/- mice, the expression of several genes of lipid oxidation are up-regulated, whereas lipid synthesis genes are down-regulated. These observations suggest that a consequence of SCD1 deficiency is an activation of lipid oxidation in addition to reduced triglyceride synthesis and storage.

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Figures

Fig 1.
Fig 1.
Body weight of male and female wild-type and SCD1−/− mice fed a chow or high-fat diet.
Fig 2.
Fig 2.
(A) Abdominal view of the fat pad under the skin in 23-week-old male wild-type and SCD1−/− mice. (B) Epididymal fat pads and liver isolated from the wild-type and SCD1−/− mice on a chow diet. (C) Epididymal fat pads and liver isolated from the wild-type and SCD1−/− mice on a high-fat diet. (Bar = 1 cm.) (D) Fat pad weights from mice fed chow and high-fat diets.
Fig 3.
Fig 3.
(A) Metabolic rate and oxygen consumption of male mice on a chow diet. (B) Gender-adjusted, normalized total oxygen consumption over a 23-h period. Error bars denote SE.
Fig 4.
Fig 4.
(A) Expression levels of lipid oxidation (Left) and lipid synthesis (Right) genes between wild-type and SCD1−/− mice. (B) Quantitative reverse-transcription–PCR of FIAF and FAS gene expression, relative to wild-type mice. 18S RNA was used as a normalization control. (C) Northern blot analysis of lipid oxidation genes and lipid synthesis genes (SREBP-1, FAS, and GPAT) in the wild-type and SCD1−/− mice.
Fig 5.
Fig 5.
Plasma glucose levels during the glucose tolerance test of male and female wild-type and SCD1−/− mice.
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Comment in

References

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