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. 2002 Apr;13(2):129-31.
doi: 10.1080/10425170290030060.

Nucleotide sequences of the internal transcribed spacers and 5.8S region of nuclear ribosomal DNA in Pinus taeda L. and Pinus echinata Mill

Jiwang Chen  1 , Charles G Tauer, Yinghua Huang
Affiliations

Nucleotide sequences of the internal transcribed spacers and 5.8S region of nuclear ribosomal DNA in Pinus taeda L. and Pinus echinata Mill

Jiwang Chen et al. DNA Seq. 2002 Apr.

Abstract

The 615-bp nucleotide sequences of the first partial internal transcribed spacer (ITS-1), 5.8S rDNA and ITS-2 region from Pinus taeda L. (loblolly pine) and Pinus echinata Mill. (shortleaf pine) are reported. The two pine species show the same nucleotide sequences in this region, which indicates their close phylogenetic relationship. However, our reported nucleotide sequence in this region from shortleaf pine is different from the previous report for shortleaf pine in GenBank (accession number: AF037016). Our PCR-RFLP analysis of this region confirms our sequencing data. This correction is important for pine phylogenetic studies because it is located in the conserved 5.8S rDNA region.

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