[The study of bacterial glycopolymers using laser spectroscopy]

Abstract

A possibility has been demonstrated to use laser spectroscopy of bacterial glycopolymers by means of measurement of their water solutions fluorescence. Comparative investigations of native lipopolysaccharide (LPS) Ralstonia solanacearum and its structure components permits a supposition to be made that the LPS total spectrum is a result of superposition of the spectrum of O-specific polysaccharide and core oligosaccharide as well as core oligosaccharide and lipid A. The LPS spectrum maximum shift is determined by core oligosaccharide and lipid A luminescence contribution. A decrease as well as complete loss of serological activity as a result of 30 and 60 min UV irradiation of LPS has been established. It has been shown that LPS Rhizobium leguminosarum bv. viciae quenches luminescence of host-plant (pea) lectin depending on the extent of their affinity. Luminescence spectrum of glucan Sinorhizobium meliloti CXM1-188 and two its LPS-mutants differ between themselves both in luminescence intensity and in presence and expression degree of the site 2 with maximum 2.8 eV.