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. 2002 Sep;68(9):4441-7.
doi: 10.1128/AEM.68.9.4441-4447.2002.

Effect of modified atmosphere composition on the metabolism of glucose by Brochothrix thermosphacta

Carmen Pin  1 , Gonzalo D García de Fernando, Juan A Ordóñez
Affiliations

Effect of modified atmosphere composition on the metabolism of glucose by Brochothrix thermosphacta

Carmen Pin et al. Appl Environ Microbiol. 2002 Sep.

Abstract

The influence of atmosphere composition on the metabolism of Brochothrix thermosphacta was studied by analyzing the consumption of glucose and the production of ethanol, acetic and lactic acids, acetaldehyde, and diacetyl-acetoin under atmospheres containing different combinations of carbon dioxide and oxygen. When glucose was metabolized under oxygen-free atmospheres, lactic acid was one of the main end products, while under atmospheres rich in oxygen mainly acetoin-diacetyl was produced. The proportions of the total consumed glucose used for the production of acetoin (aerobic metabolism) and lactic acid (anaerobic metabolism) were used to decide whether aerobic or anaerobic metabolism predominated at a given atmosphere composition. The boundary conditions between dominantly anaerobic and aerobic metabolisms were determined by logistic regression. The metabolism of glucose by B. thermosphacta was influenced not only by the oxygen content of the atmosphere but also by the carbon dioxide content. At high CO(2) percentages, glucose metabolism remained anaerobic under greater oxygen contents.

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Figures

FIG. 1.
FIG. 1.
Growth (•); production of lactic acid (×), acetoin-diacetyl (□), acetic acid (○), and ethanol (◊); and consumption of glucose (▵) by B. thermosphacta at 5°C under atmospheres with 0/80/20 (a) and 0/0/100 (b) ratios of CO2-O2-N2, respectively.
FIG. 2.
FIG. 2.
Lactic acid production rate (a), acetoin-diacetyl production rate (b), total amount of acetic acid (c), glucose consumption rate (d), and maximum specific growth rate (e) of B. thermosphacta at 5°C and different percentages of oxygen and carbon dioxide in the atmosphere.
FIG. 3.
FIG. 3.
Proportion of glucose consumed aerobically (a) and anaerobically (b) under different percentages of oxygen and carbon dioxide in the atmosphere.
FIG. 4.
FIG. 4.
Atmosphere compositions that determine the boundary between dominantly aerobic (○) or dominantly anaerobic (•) metabolism in B. thermosphacta.
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References

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