Assessment of techniques used in calculating cell-material interactions

Abstract

A review of the techniques used in measuring the forces of deadhesion of cells that have been adhering on substrate surfaces is presented. Two categories of techniques are described, those that utilize fluid flowing against the adhered cells and counting the percentage of cells that detach (global tests) and the manipulation of single cells in various configurations which lend themselves to more specific force application and provide the basis for theoretical analysis of the receptor-ligand mechanics.