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. 1977 Jun;115(6):1066-9.
doi: 10.1164/arrd.1977.115.6.1066.

Automatable radiometric detection of growth of Mycobacterium tuberculosis in selective media

G Middlebrook, Z Reggiardo, W D Tigertt

Automatable radiometric detection of growth of Mycobacterium tuberculosis in selective media

G Middlebrook et al. Am Rev Respir Dis. 1977 Jun.

Abstract

The formulation of media for selective, automatable, radiometric detection of growth of Mycobacterium tuberculosis in vitro is described. Palmitic-1 acid labeled with carbon-14 and formic-14C acid were compared as substrate sources of [14C]O2 in media deficient in carbohydrate and containing appropriate antimicrobial agents that are not active against tubercle bacilli. A preliminary clinical laboratory study of a medium containing 4 microCi palmitic-1-14C acid per ml showed that this method might provide the basis for practical laboratory use.

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