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Case Reports
. 2002 Sep 10:2:19.
doi: 10.1186/1471-2334-2-19. Epub 2002 Sep 10.

Impact of HIV vaccination on laboratory diagnosis: case reports

Vongsheree Suthon  1 , Rojanawiwat Archawin, Chardbanchachai Chanchai, Lerwitworapong John, Kongpromsook Wichuda, Paungtubtim Wiroj, Thaisri Hansa, Sawanpanyalert Pathom, Sri-ngam Pongnuwat, Pithak Silaporn, Inunchot Wimala
Affiliations
Case Reports

Impact of HIV vaccination on laboratory diagnosis: case reports

Vongsheree Suthon et al. BMC Infect Dis. .

Abstract

Background: It has not been clearly demonstrated whether HIV vaccination can complicate routine HIV testing. In this report, we describe the laboratory data of two prisoners who received rgp120 vaccine in a phase III trial underway in Thailand. These data indicate that previous vaccination may complicate the interpretation of screening HIV diagnostic tests.

Case presentation: The participants were identified from a cohort study on "Health factors related to HIV-1 and other viral infections among incarcerated people" that was approved by The Ethical Committee for Research in Human Subjects, Ministry of Public Health, Thailand. HIV diagnosis was definitively established with serial specimens using multi-screening tests, Western blot and diagnostic PCR.Anti-HIV screening tests consistently exhibited either weakly reactive or inconclusive results. The band patterns of the Western blot analysis corresponded to those found in individuals who received the rgp120 vaccination. Definite results were established using diagnostic PCR, which exhibited consistently negative results with follow-up specimens. Such problems in HIV testing are not easily resolved in the routine clinical setting in Thailand.

Conclusions: These data demonstrate that HIV-1 vaccination interferes with routine diagnostic tests. Similar cases will not be uncommon in Thailand, where 2,545 people have already participated in a phase III trial.

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Figures

Figure 1
Figure 1
Western Blot results of KP 548 and KP667 *Day after first visit. WPC: weak positive control
See this image and copyright information in PMC

References

    1. Francis DP, Gregory T, McElrath MJ, Belshe RB, Gorse GJ, Migasena S, Kitayaporn D, Pitisuttitham P, Matthews T, Schwartz DH, Berman PW. Advancing AIDSVAX to phase 3. Safety, immunogenicity, and plan for phase 3. AIDS Res Hum Retroviruses. 1998;14:S325–S331. - PubMed
    1. The HIV/AIDS Collaboration, Thailand Annual Report for 2000–2001. Part C. HIV transmission among injecting drug users and HIV vaccine efficacy trial. pp. 30–35.
    1. Phanupak P, Teeratakulpixarn S, Sarangbin S, Nookhai S, Ubolyam S, Sirivichayabul S, Leesavan A, Forrest BD, Hanson CV, Li M, Wang CY, et al. International clinical trials of HIV vaccines: I. Phase I trial of an HIV-1 synthetic peptide vaccine in Bangkok, Thailand. Asian Pac J Allergy Immunol. 1997;15:41–48. - PubMed
    1. Migasena S, Suntharasamai P, Pitisuttitham P, Kitayaporn D, Wasi C, Huang W, Vanichseni S, Koompong C, Kaewkungwal J, Raktham S, et al. AIDSVAX (MN) in Bangkok injecting drug users: a report on safety and immunogenicity, including macrophage-tropic virus neutralization. AIDS Res Hum Retrovirus. 2000;16:655–663.41-48. doi: 10.1089/088922200308882. - DOI - PubMed
    1. Allen M, Israel H, Rybezyk K, Pugliese MA, Loughran K, Wagner L, Erb S. Trial-related discrimination in HIV vaccine clinical trials. AIDS RES Hum Retroviruses. 2001;17:667–674. doi: 10.1089/088922201750236942. - DOI - PubMed

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