Changes in Lipid Peroxidation and Lipolytic and Free-Radical Scavenging Enzyme Activities during Aging and Sprouting of Potato (Solanum tuberosum) Seed-Tubers

Abstract

Previous research has shown that cell membranes of potato (Solanum tuberosum L. cv Russet Burbank) seed-tubers lose integrity between 7 and 26 months of storage (4[deg]C, 95% relative humidity), and this loss coincides with a significant decrease in growth potential. The age-induced decline in membrane integrity is apparently due to increased peroxidative damage of membrane lipids. Malondialdehyde (MDA) and ethane concentrations (sensitive markers of lipid peroxidation and membrane damage) increased in seed-tuber tissues with advancing age. Moreover, in vivo ethane production from discs of cortex tissue from 13- and 25-month-old seed-tubers was 87% greater (on average) than that from discs from 1-month-old tubers. Calcium suppressed ethane production from all ages of tissue discs, and the effect was concentration dependent. Linoleic acid enhanced ethane production from 5- and 17-month-old tubers by 61 and 228%, respectively, suggesting that older tissue may contain a higher free-radical (FR) titer and/or lower free polyunsaturated fatty acid content. In addition, throughout plant establishment, the internal ethane concentration of older seed-tubers was 54% higher than that of younger seed-tubers. MDA concentration of tuber tissue declined by about 65% during the initial 7 months of storage and then increased 267% as tuber age advanced to 30 months. The age-induced trend in tuber reducing sugar concentration was similar to that of MDA, and the two were linearly correlated. The age-dependent increase in reducing sugars may thus reflect peroxidative degeneration of the amyloplast membrane, leading to increased starch hydrolysis. Compared with 5-month-old seed tubers, 17- and 29-month-old seed-tubers had significantly higher levels of lipofuscin-like fluorescent compounds (FCs), which are produced when MDA reacts with free amino acids. Age-dependent increases in MDA, ethane, and FCs were not associated with higher activities of phospholipase and lipoxygenase in tissue from older tubers. In fact, 8-month-old seed-tubers had significantly higher activities of these enzymes than 20-month-old seed-tubers. However, the activities of superoxide dismutase, peroxidase, and catalase in 20-month-old tubers were substantially higher out of storage, and increased at a faster rate during plant establishment, than in 8-month-old seed-tubers. Collectively, these results suggest that a gradual build-up of FRs leads to peroxidative damage of membrane lipids during aging of potato seed-tubers.