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. 1994 Apr;104(4):1381-1384.
doi: 10.1104/pp.104.4.1381.

Phosphoinositides in Barley (Hordeum vulgare L.) Aleurone Tissue

C. A. Brearley  1 , D. E. Hanke
Affiliations

Phosphoinositides in Barley (Hordeum vulgare L.) Aleurone Tissue

C. A. Brearley et al. Plant Physiol. 1994 Apr.

Abstract

[3H]Inositol labeling of barley (Hordeum vulgare L. cv Himalaya) aleurone layers and analysis of phospholipids by deacylation revealed the presence of phosphatidylinositol (PtdIns), PtdIns3P, and PtdIns4P but not PtdInsP2 species. In contrast to an earlier report (P.P.N. Murthy, G. Pliska-Matyshak, L.M. Keranen, P. Lam, H.H. Mueller, N. Bhuvarahamurthy [1992] Plant Physiol 98: 1498-1501) systematic chemical degradation of PtdIns revealed no evidence of a second isomer of PtdIns. Evidence of the widespread occurrence of 3-phosphorylated PtdIns within the plant kingdom is presented.

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