Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 Oct;46(10):3304-7.
doi: 10.1128/AAC.46.10.3304-3307.2002.

Simple method for assaying colistin methanesulfonate in plasma and urine using high-performance liquid chromatography

Jian Li  1 , Robert W Milne, Roger L Nation, John D Turnidge, Kingsley Coulthard, Jason Valentine
Affiliations

Simple method for assaying colistin methanesulfonate in plasma and urine using high-performance liquid chromatography

Jian Li et al. Antimicrob Agents Chemother. 2002 Oct.

Abstract

A simple and sensitive high-performance liquid chromatographic method is described for the determination of colistimethate sodium in plasma and urine. The accuracy and reproducibility was within 10.1 and 11.2% with rat plasma and urine, respectively. Several commonly coadministered antibacterial agents do not interfere with the assay.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Effects of the concentration of sulfuric acid (a) and time (b) on the hydrolysis of CMS to colistin in rat plasma. Response is expressed as a percentage of the maximum observed response.
FIG. 2.
FIG. 2.
Chromatogram of a rat plasma sample collected 10 min after an intravenous bolus of 15 mg of CMS/kg. Net, Col B, Col A, peaks of netilmicin, colistin A, and colistin B, respectively.
See this image and copyright information in PMC

References

    1. Banerjee, D., and D. Stableforth. 2000. The treatment of respiratory pseudomonas infection in cystic fibrosis: what drug and which way? Drugs 60:1053-1064. - PubMed
    1. Barnett, M., S. R. Bushby, and S. Wilkinson. 1964. Sodium sulphomethyl derivatives of polymyxins. Br. J. Pharmacol. 23:552-574. - PMC - PubMed
    1. Beveridge, E. G., and A. J. Martin. 1967. Sodium sulphomethyl derivatives of polymyxins. Br. J. Pharmacol. Chemother. 29:125-135. - PMC - PubMed
    1. Decolin, D., P. Leroy, A. Nicolas, and P. Archimbault. 1997. Hyphenated liquid chromatographic method for the determination of colistin residues in bovine tissues. J. Chromatogr. Sci. 35:557-564. - PubMed
    1. Evans, M. E., D. J. Feola, and R. P. Rapp. 1999. Polymyxin B sulfate and colistin: old antibiotics for emerging multiresistant gram-negative bacteria. Ann. Pharmacother. 33:960-967. - PubMed

Publication types

LinkOut - more resources