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. 2002 Oct;46(10):3334-6.
doi: 10.1128/AAC.46.10.3334-3336.2002.

Gene dosage and linezolid resistance in Enterococcus faecium and Enterococcus faecalis

S H Marshall  1 , C J Donskey, R Hutton-Thomas, R A Salata, L B Rice
Affiliations

Gene dosage and linezolid resistance in Enterococcus faecium and Enterococcus faecalis

S H Marshall et al. Antimicrob Agents Chemother. 2002 Oct.

Abstract

Resistance to linezolid has been associated with a G2576U mutation in domain V of the 23S rRNA. We analyzed nine clinical isolates of linezolid-resistant enterococci and showed a clear association between the number of 23S rRNA genes containing this mutation and the level of linezolid resistance expressed.

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Figures

FIG. 1.
FIG. 1.
(A) Diagrammatic representation of the amplification and restriction strategy employed to assess the number of 23S rRNA genes. The thin line represents the PCR amplification product. The thick line represents the multiple cloning site of the pCR-XL-TOPO vector. The arrows represent the forward and reverse primers used to generate the PCR product containing the 23S insert and the multiple cloning site of the vector. MaeI sites within the insert (created by the presence of the G2576T mutation) and within the vector multiple cloning site are indicated. As the figure indicates, the size of the restriction fragments resulting from MaeI digestion of the PCR products will differ depending on the orientation of the insert. (B) MaeI digestion of cloned amplification products as described for panel A. Two additional bands are readily apparent in lanes with inserts containing the G2576T mutation (lanes marked by asterisks). Linezolid MICs for the strains are indicated above the panels and in Table 1.
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References

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