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. 2002 Oct;9(10):725-8.
doi: 10.1038/nsb844.

L-A virus at 3.4 A resolution reveals particle architecture and mRNA decapping mechanism

Hisashi Naitow  1 , Jinghua Tang, Mary Canady, Reed B Wickner, John E Johnson
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L-A virus at 3.4 A resolution reveals particle architecture and mRNA decapping mechanism

Hisashi Naitow et al. Nat Struct Biol. 2002 Oct.

Abstract

The structure of the yeast L-A virus was determined by X-ray crystallography at 3.4 A resolution. The L-A dsRNA virus is 400 A in diameter and contains a single protein shell of 60 asymmetric dimers of the coat protein, a feature common among the inner protein shells of dsRNA viruses and probably related to their unique mode of transcription and replication. The two identical subunits in each dimer are in non-equivalent environments and show substantially different conformations in specific surface regions. The L-A virus decaps cellular mRNA to efficiently translate its own uncapped mRNA. Our structure reveals a trench at the active site of the decapping reaction and suggests a role for nearby residues in the reaction.

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