An improved synthetic medium suitable for tissue culture of various mammalian cells

Abstract

A new mixture of synthetic medium, DM-153, was designed to obtain a higher rate of proliferation of various cells and to be adoptable for both of closed and opened systems of culture. The activity was compared to that of other synthetic media by cultivation for 2 weeks. In the presence of 10% (v/v) fetal calf serum, DM-153 exhibited the highest rate of proliferation of rat liver cells, strain RLC-10(2), than did Eagle MEM, DM-145 or lactalbumin hydrolysate which had been employed as a routine growth medium for the cells. In the absence of any high molecular weight substances in the medium, both of DM-153 and DM-145 showed higher rate of proliferation of rat liver cells, strain JTC-25-P3, than that obtained by MEM. In the case of primary culture of human blood lymphoid cells supplemented with 10% fetal calf serum, DM-153 resulted in apparently higher rate of cell survival than did RPMI-1640. The mixture DM-153 was confirmed to be suitable as a multi-purpose synthetic medium.