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. 2002 Sep;13(9):1129-37.
doi: 10.1016/S1044-0305(02)00430-0.

Additives for the stabilization of double-stranded DNA in UV-MALDI MS

Anne M Distler  1 , John Allison
Affiliations

Additives for the stabilization of double-stranded DNA in UV-MALDI MS

Anne M Distler et al. J Am Soc Mass Spectrom. 2002 Sep.

Abstract

Molecular complexes such as double-stranded oligonucleotides contain non-covalent bonds that are difficult to maintain in the MALDI experiment. Quantifiers are introduced in order to evaluate, summarize, and compare spectra from experiments in which additives are used to stabilize duplex oligonucleotides. Compounds known to complex with and stabilize duplex molecules can be useful as additives in MALDI. Spermine and methylene blue, present at concentrations similar to the matrix, are detected, bound to the duplex. When peptides are used as additives, the duplex is stabilized when the peptide is present at an amount less than that of the duplex.

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References

    1. Biochemistry. 1998 Jun 9;37(23):8341-55 - PubMed
    1. Anal Chem. 2000 Aug 15;72(16):3860-6 - PubMed
    1. Biochemistry. 1991 Dec 3;30(48):11388-402 - PubMed
    1. Rapid Commun Mass Spectrom. 1996;10(8):907-10 - PubMed
    1. Nucleic Acids Res. 1998 Jul 1;26(13):3136-45 - PubMed

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