Enzyme activation for nonaqueous media

Abstract

Highly active enzyme formulations can be prepared for use in nonaqueous media. Considerable progress has been made in the past two years on gaining an improved mechanistic understanding of enzyme function and activation in dehydrated environments. This increased fundamental understanding has led to the development of a broad array of techniques for generating active, stable, and enantioselective and regioselective tailored enzymes for synthetically relevant transformations. This, in turn, is resulting in an exponential increase in the opportunities for enzymatic processes to be developed on a commercial scale.