Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Jun 13;8(5):359-63.
doi: 10.1007/BF00562663.

Assay of the major (4-hydroxylated) metabolites of diphenylhydantoin in human urine

B Karlén, M Garle, A Rane, M Gutova, B Lindborg

Assay of the major (4-hydroxylated) metabolites of diphenylhydantoin in human urine

B Karlén et al. Eur J Clin Pharmacol. .

Abstract

A modified gas chromatographic procedure for the determination of unconjugated and conjugated 4-hydroxydiphenylhydantoin (4-OH-DPH) in urine has been developed. Unconjugated 4-OH-DPH is determined after selective extraction with toluene-ether (1:1). For the assay of conjugated 4-OH-DPH, the urine is pre-extracted with isoamylalcohol before acid hydrolysis to avoid interference by the dihydrodiol metabolite of DPH. The sensitivity of the method is 0.1 mug per ml. The method has been used to determine the urinary metabolites in two adult volunteers, during steady state plasma concentrations of DPH and in the elimination phase.

PubMed Disclaimer

References

    1. J Pharmacol Exp Ther. 1957 Jan;119(1):1-11 - PubMed
    1. Scand J Clin Lab Invest. 1972 May;29(3):281-7 - PubMed
    1. J Pharmacol Exp Ther. 1960 Nov;130:275-84 - PubMed
    1. Res Commun Chem Pathol Pharmacol. 1972 Jul;4(1):13-23 - PubMed
    1. Arch Int Pharmacodyn Ther. 1973 May;203(1):72-91 - PubMed

LinkOut - more resources