Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 Oct;40(10):3793-7.
doi: 10.1128/JCM.40.10.3793-3797.2002.

Use of 16S rRNA gene sequencing for identification of nonfermenting gram-negative bacilli recovered from patients attending a single cystic fibrosis center

Agnes Ferroni  1 , Isabelle Sermet-Gaudelus, Eric Abachin, Gilles Quesne, Gerard Lenoir, Patrick Berche, Jean-Louis Gaillard
Affiliations

Use of 16S rRNA gene sequencing for identification of nonfermenting gram-negative bacilli recovered from patients attending a single cystic fibrosis center

Agnes Ferroni et al. J Clin Microbiol. 2002 Oct.

Abstract

During 1999, we used partial 16S rRNA gene sequencing for the prospective identification of atypical nonfermenting gram-negative bacilli isolated from patients attending our cystic fibrosis center. Of 1,093 isolates of nonfermenting gram-negative bacilli recovered from 148 patients, 46 (4.2%) gave problematic results with conventional phenotypic tests. These 46 isolates were genotypically identified as Pseudomonas aeruginosa (19 isolates, 12 patients), Achromobacter xylosoxidans (10 isolates, 8 patients), Stenotrophomonas maltophilia (9 isolates, 9 patients), Burkholderia cepacia genomovar I/III (3 isolates, 3 patients), Burkholderia vietnamiensis (1 isolate), Burkholderia gladioli (1 isolate), and Ralstonia mannitolilytica (3 isolates, 2 patients), a recently recognized species.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Identification scheme used during the study.
FIG. 2.
FIG. 2.
Position of CF Burkholderia and Ralstonia isolates. The bar indicates 10% estimated sequence divergence. Clinical isolates are designated by their laboratory number.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Bauernfeind, A., I. Schneider, R. Jungwirth, and C. Roller. 1998. Discrimination of Burkholderia gladioli from other Burkholderia species detectable in cystic fibrosis patients by PCR. J. Clin. Microbiol. 36:2748-2751. - PMC - PubMed
    1. Bauernfeind, A., I. Schneider, R. Jungwirth, and C. Roller. 1999. Discrimination of Burkholderia multivorans and Burkholderia vietnamiensis from Burkholderia cepacia genomovars I, III, and IV by PCR. J. Clin. Microbiol. 37:1335-1339. - PMC - PubMed
    1. Burdge, D. R., M. A. Noble, M. E. Campbell, V. L. Krell, and D. P. Speert. 1995. Xanthomonas maltophilia misidentified as Pseudomonas cepacia in cultures of sputum from patients with cystic fibrosis: a diagnostic pitfall with major clinical implications. Clin. Infect. Dis. 2:445-448. - PubMed
    1. Burns, J. L., J. Emerson, J. R. Stapp, D. L. Yim, Y. J. Krzewinski, L. Louden, B. W. Ramsey, and C. R. Clausen. 1998. Microbiology of sputum from patients at cystic fibrosis centers in the United States. Clin. Infect. Dis. 27:158-163. - PubMed
    1. Clode, F. E., M. E. Kaufmann, H. Malnick, and T. L. Pitt. 1999. Evaluation of three oligonucleotide primer sets in PCR for the identification of Burkholderia cepacia and their differentiation from Burkholderia gladioli. J. Clin. Pathol. 52:173-176. - PMC - PubMed

MeSH terms

Substances

LinkOut - more resources