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. 2002 Oct;40(10):3793-7.
doi: 10.1128/JCM.40.10.3793-3797.2002.

Use of 16S rRNA gene sequencing for identification of nonfermenting gram-negative bacilli recovered from patients attending a single cystic fibrosis center

Agnes Ferroni  1 , Isabelle Sermet-Gaudelus, Eric Abachin, Gilles Quesne, Gerard Lenoir, Patrick Berche, Jean-Louis Gaillard
Affiliations

Use of 16S rRNA gene sequencing for identification of nonfermenting gram-negative bacilli recovered from patients attending a single cystic fibrosis center

Agnes Ferroni et al. J Clin Microbiol. 2002 Oct.

Abstract

During 1999, we used partial 16S rRNA gene sequencing for the prospective identification of atypical nonfermenting gram-negative bacilli isolated from patients attending our cystic fibrosis center. Of 1,093 isolates of nonfermenting gram-negative bacilli recovered from 148 patients, 46 (4.2%) gave problematic results with conventional phenotypic tests. These 46 isolates were genotypically identified as Pseudomonas aeruginosa (19 isolates, 12 patients), Achromobacter xylosoxidans (10 isolates, 8 patients), Stenotrophomonas maltophilia (9 isolates, 9 patients), Burkholderia cepacia genomovar I/III (3 isolates, 3 patients), Burkholderia vietnamiensis (1 isolate), Burkholderia gladioli (1 isolate), and Ralstonia mannitolilytica (3 isolates, 2 patients), a recently recognized species.

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Figures

FIG. 1.
FIG. 1.
Identification scheme used during the study.
FIG. 2.
FIG. 2.
Position of CF Burkholderia and Ralstonia isolates. The bar indicates 10% estimated sequence divergence. Clinical isolates are designated by their laboratory number.
See this image and copyright information in PMC

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