DNA differential diagnosis of human taeniid cestodes by base excision sequence scanning thymine-base reader analysis with mitochondrial genes

Abstract

For DNA differential diagnosis of human Taenia cestodes, a base excision sequence scanning thymine-base method using the cytochrome c oxidase subunit I and cytochrome b genes as targets was used. The characteristic thymine-base peak profiles provide four distinct types, unique for T. saginata, T. asiatica, and two genotypes of T. solium. This approach provides a useful tool for the identification and diagnosis of human taeniid cestodes without DNA sequencing if nucleotide sequence databases are available.

FIG. 1.

BESS-T Base Reader analysis with Cox 1 (A) and Cytb (B) fragments. Profiles from T. saginata (China), T. asiatica (Taiwan), and T. solium (Brazil, Tanzania, and China) are shown. The nucleotide sequences indicated above the peaks are from the databases listed in Table 1. Diagnostic positions are denoted by arrows.