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. 2002 Oct;40(10):3818-21.
doi: 10.1128/JCM.40.10.3818-3821.2002.

DNA differential diagnosis of human taeniid cestodes by base excision sequence scanning thymine-base reader analysis with mitochondrial genes

Hiroshi Yamasaki  1 , Minoru Nakao, Yasuhito Sako, Kazuhiro Nakaya, Marcello Otake Sato, Wulamu Mamuti, Munehiro Okamoto, Akira Ito
Affiliations

DNA differential diagnosis of human taeniid cestodes by base excision sequence scanning thymine-base reader analysis with mitochondrial genes

Hiroshi Yamasaki et al. J Clin Microbiol. 2002 Oct.

Abstract

For DNA differential diagnosis of human Taenia cestodes, a base excision sequence scanning thymine-base method using the cytochrome c oxidase subunit I and cytochrome b genes as targets was used. The characteristic thymine-base peak profiles provide four distinct types, unique for T. saginata, T. asiatica, and two genotypes of T. solium. This approach provides a useful tool for the identification and diagnosis of human taeniid cestodes without DNA sequencing if nucleotide sequence databases are available.

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Figures

FIG. 1.
FIG. 1.
BESS-T Base Reader analysis with Cox 1 (A) and Cytb (B) fragments. Profiles from T. saginata (China), T. asiatica (Taiwan), and T. solium (Brazil, Tanzania, and China) are shown. The nucleotide sequences indicated above the peaks are from the databases listed in Table 1. Diagnostic positions are denoted by arrows.
See this image and copyright information in PMC

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