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Comparative Study
. 2002 Oct;40(10):3851-3.
doi: 10.1128/JCM.40.10.3851-3853.2002.

Use of spoligotyping for accurate classification of recurrent tuberculosis

R M Warren  1 , E M Streicher, S Charalambous, G Churchyard, G D van der Spuy, A D Grant, P D van Helden, T C Victor
Affiliations
Comparative Study

Use of spoligotyping for accurate classification of recurrent tuberculosis

R M Warren et al. J Clin Microbiol. 2002 Oct.

Abstract

The spoligotyping method has become an important tool for the tracking of Mycobacterium tuberculosis strains in different epidemiological settings. In this study, we demonstrate the ability of the spoligotyping technique to accurately determine the pathogenetic mechanism of recurrent disease. This methodology has advantages over conventional restriction fragment length polymorphism methods which may be useful in large-scale intervention studies.

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Figures

FIG. 1.
FIG. 1.
IS6110 RFLP and spoligotype analysis of clinical isolates collected from different episodes of disease from patients with recurrent disease. (A) IS6110 RFLP analysis. Pairs of lanes under a single lane number represent the isolates taken from a single patient but different episodes of disease. Lanes 1 and 2 show that the results for the two episodes represented in each pair of lanes are genotypically identical for each of those patients, demonstrating relapse, while lanes 3 and 4 show that the results for the two episodes represented in each pair of lanes are genotypically different for each of those patients, demonstrating reinfection. (B) Spoligotype analysis. Each pair of isolates represented corresponds to the isolates represented under the same lane number in panel A. Lanes 1 and 2 show that the results for the two episodes represented in each pair of lanes are genotypically identical for each of those patients, demonstrating relapse, while lanes 3 and 4 show that the results for the two episodes represented in each pair of lanes are genotypically different for each of those patients, demonstrating reinfection.
See this image and copyright information in PMC

References

    1. Bauer, J., A. B. Andersen, K. Kremer, and H. Miorner. 1999. Usefulness of spoligotyping to discriminate IS6110 low-copy-number Mycobacterium tuberculosis complex strains cultured in Denmark. J. Clin. Microbiol. 37:2602-2606. - PMC - PubMed
    1. Corbett, E. L., G. J. Churchyard, T. Clayton, P. Herselman, B. Williams, R. Hayes, D. Mulder, and K. M. De Cock. 1999. Risk factors for pulmonary mycobacterial disease in South African gold miners. A case-control study. Am. J. Respir. Crit. Care Med. 159:94-99. - PubMed
    1. Cronin, W. A., J. E. Golub, L. S. Magder, N. G. Baruch, M. J. Lathan, L. N. Mukasa, N. Hooper, J. H. Razeq, D. Mulcahy, W. H. Benjamin, and W. R. Bishai. 2001. Epidemiologic usefulness of spoligotyping for secondary typing of Mycobacterium tuberculosis isolates with low copy numbers of IS6110. J. Clin. Microbiol. 39: 3709-3711. - PMC - PubMed
    1. de C. Ramos, M., H. Soini, G. C. Roscanni, M. Jaques, M. C. Villares, and J. M. Musser. 1999. Extensive cross-contamination of specimens with Mycobacterium tuberculosis in a reference laboratory. J. Clin. Microbiol. 37:916-919. - PMC - PubMed
    1. Fang, Z., N. Morrison, B. Watt, C. Doig, and K. J. Forbes. 1998. IS6110 transposition and evolutionary scenario of the direct repeat locus in a group of closely related Mycobacterium tuberculosis strains. J. Bacteriol. 180:2102-2109. - PMC - PubMed

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