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. 2002 Oct;40(10):3857-9.
doi: 10.1128/JCM.40.10.3857-3859.2002.

Molecular detection of human calicivirus among Spanish children with acute gastroenteritis

E Roman  1 , A Negredo, R M Dalton, I Wilhelmi, A Sánchez-Fauquier
Affiliations

Molecular detection of human calicivirus among Spanish children with acute gastroenteritis

E Roman et al. J Clin Microbiol. 2002 Oct.

Abstract

A survey was conducted among Spanish children with gastroenteritis treated in an emergency room. Reverse transcription-PCR with specimens negative for other enteric pathogens was used. The minimum incidence of human calicivirus infection was 7.7%, with Lordsdale as the predominant genotype. The clinical features and severity of calicivirus and rotavirus were similar.

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Figures

FIG. 1.
FIG. 1.
Phylogenic tree based on analyses of a 236-bp region of the RNA polymerase gene of three Spanish strains of SLV obtained in this study. Distances were calculated by the Kimura 2p method, available in the MEGA, version 2.1, analytical package. The tree was drawn by using the neighbor-joining method. Bootstrap values for each node are given if they are >80%. Genogroups and genetic clusters (GC) were named according to the Schuffenecker study of genetic classifications of SLVs (18). GenBank accession numbers for the SLV sequences used in this study are as follows: Lyon/98, AJ251991; Sapporo/82, U65427; Manchester/93, X86560; Houston/86, U95643; Houston/90, U95644; Parkville/94, U73124; Lyon/97, AJ271056; London/92, U95645. Spanish strains are marked in bold.
FIG. 2.
FIG. 2.
Monthly distribution of HuCV-associated cases as percentages of HuCV-positive samples.
See this image and copyright information in PMC

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