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. 2002;16(5):233-6.
doi: 10.1002/jcla.10049.

Spectrophotometric determination of leukocytes in blood

Huriye Kuzu-Karsilayan  1 , Eda Eryilmaz, Gaye Yillar, Günnur Deniz, Gülderen Yanikkaya-Demirel
Affiliations

Spectrophotometric determination of leukocytes in blood

Huriye Kuzu-Karsilayan et al. J Clin Lab Anal. 2002.

Abstract

The determination of leukocyte concentration in human blood depending on the detection of oxidized o-dianisidine in acidic solution is studied. The oxidation of o-dianisidine was carried out by peroxidase enzymes found in leukocytes. The reaction was stopped by the addition of 4N H(2)SO(4) to the solution, and a very stable, colored o-dianisidine derivative was obtained. The calibration graph was plotted with the recorded absorbance values at 400 nm assigned to the y-axis, and leukocyte counts in 1-mL blood samples to the x-axis. The equation of the calibration graph was y=0.0025 x +0.0904, with a correlation coefficient of R=0.994. The coefficient of variation and P-value of the method were 4.00% and 0.05%, respectively.

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References

    1. Thomas EL, Fishman M. 1986. Oxidation of chloride and thiocynate by isolated leukocytes. J Biol Chem 261:9694–702. - PubMed
    1. Harrison JE, Schultz J. 1976. Studies on the chlorinating activity of myeloperoxidase. J Biol Chem 251:1371–1374. - PubMed
    1. Foote CS, Goyne TE, Lehrer RI. 1983. Assessment of chlorination by human neutrophils. Nature 301:715–716. - PubMed
    1. Schneider T, Issekutz AC. 1996. Quantitation of eosinophil and neutrophil infiltration into rat lung by specific assays for eosinophil peroxidase and myeloperoxidase. J Immunol Methods 198:1–14. - PubMed
    1. Andrews PC, Krinsky NI. 1982. A kinetic analysis of the interaction of human myeloperoxidase with hydrogen peroxide, chlorine ions and protons. J Biol Chem 257:13240–13245. - PubMed

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