doi: 10.1007/BF01538667.
Chinese hamster X mouse hybrid cells segregating mouse chromosomes and isozymes
- PMID: 1235911
- DOI: 10.1007/BF01538667
Item in Clipboard
Chinese hamster X mouse hybrid cells segregating mouse chromosomes and isozymes
Somatic Cell Genet.
1975 Oct.
Abstract
Hybrid cells are readily formed by fusing clonal Chinese hamster cells to fresh, noncultured, adult mouse spleen cells followed by isolation in selective medium. The vast majority of such hybrids retain Chinese hamster chromosomes and isozymes while segregating mouse chromosomes and isozymes. The growth, plating efficiency, ease of karyology, and rapid segregation of mouse markers allows linkage tests in primary clones. Analysis of 13 isozymes showed 12 to be asyntenic and on epair (PGD-PGM2) to be syntenic This system will allow extensive somatic cell hybrid gene mapping in the mouse and permit a comparison of human and mouse linkage relationships.
Similar articles
-
Proceedings: The human loci for phosphopyruvate hydratase and guanylate kinase are syntenic with the PGD-PGM1 linkage group in man-Chinese hamster somatic cell hybrids.Cytogenet Cell Genet. 1974;13(1):130-1. doi: 10.1159/000130255. Cytogenet Cell Genet. 1974. PMID: 4363865 No abstract available.
-
Evidence for linkage between human loci for 6-phosphogluconate dehydrogenase and phosphoglucomutase in man-Chinese hamster somatic cell hybrids.Nature. 1972 Mar 3;236(5340):30-2. doi: 10.1038/236030a0. Nature. 1972. PMID: 4553635 No abstract available.
-
Homologous genes for enolase, phosphogluconate dehydrogenase, phosphoglucomutase, and adenylate kinase are syntenic on mouse chromosome 4 and human chromosome 1p.Proc Natl Acad Sci U S A. 1978 May;75(5):2382-6. doi: 10.1073/pnas.75.5.2382. Proc Natl Acad Sci U S A. 1978. PMID: 209463 Free PMC article.
-
Linkage analysis using somatic cell hybrids.Adv Hum Genet. 1972;30:173-235. doi: 10.1007/978-1-4757-4429-3_4. Adv Hum Genet. 1972. PMID: 4125882 Review. No abstract available.
-
Regulation of chromosome replication and segregation in bacteria.Bacteriol Rev. 1966 Mar;30(1):3-32. doi: 10.1128/br.30.1.3-32.1966. Bacteriol Rev. 1966. PMID: 5324649 Free PMC article. Review. No abstract available.
Cited by
-
Mouse X chromosome.Mamm Genome. 1992;3 Spec No:S274-88. doi: 10.1007/BF00648438. Mamm Genome. 1992. PMID: 1498440 Review. No abstract available.
-
Molecular cloning of translocations involving chromosome 15 and the immunoglobulin C alpha gene from chromosome 12 in two murine plasmacytomas.Proc Natl Acad Sci U S A. 1982 Nov;79(22):6994-8. doi: 10.1073/pnas.79.22.6994. Proc Natl Acad Sci U S A. 1982. PMID: 6960358 Free PMC article.
-
Comparative mapping using somatic cell hybrids.In Vitro. 1976 Nov;12(11):726-33. doi: 10.1007/BF02835447. In Vitro. 1976. PMID: 828614
-
Continuous, clonal, insulin- and somatostatin-secreting cell lines established from a transplantable rat islet cell tumor.Proc Natl Acad Sci U S A. 1980 Jun;77(6):3519-23. doi: 10.1073/pnas.77.6.3519. Proc Natl Acad Sci U S A. 1980. PMID: 6106192 Free PMC article.
-
Mouse X chromosome.Mamm Genome. 1991;1 Spec No:S318-31. doi: 10.1007/BF00656501. Mamm Genome. 1991. PMID: 1799807 Review. No abstract available.
MeSH terms
Substances
LinkOut - more resources
Molecular Biology Databases
Miscellaneous