Higher order arrangement of the eukaryotic nuclear bodies
- PMID: 12361981
- PMCID: PMC129717
- DOI: 10.1073/pnas.212483099
Higher order arrangement of the eukaryotic nuclear bodies
Abstract
The nuclei of eukaryotic cells consist of discrete substructures. These substructures include the nuclear bodies, which have been implicated in a number of biological processes such as transcription and splicing. However, for most nuclear bodies, the details of involvement in these processes in relation to their three-dimensional distributions in the nucleus are still unclear. Through the analysis of TDP, a protein functional in both transcriptional repression and alternative splicing, we have identified a new category of nuclear bodies within which the TDP molecules reside. Remarkably, the TDP bodies (TBs) colocalize or overlap with several different types of nuclear bodies previously suggested to function in transcription or splicing. Of these nuclear bodies, the Gemini of coiled bodies (GEM) seems to associate with TB through the interaction between survival motor neuron (SMN) protein and TDP. Furthermore, TB sometimes appears to be the bridge of two or more of these other nuclear bodies. Our data suggest the existence of a hierarchy and possibly functional arrangement of the nuclear bodies within the eukaryotic nuclei.
Figures








Similar articles
-
"On the move"ments of nuclear components in living cells.Exp Cell Res. 2004 May 15;296(1):4-11. doi: 10.1016/j.yexcr.2004.03.018. Exp Cell Res. 2004. PMID: 15120987 Review.
-
Altered distributions of Gemini of coiled bodies and mitochondria in motor neurons of TDP-43 transgenic mice.Proc Natl Acad Sci U S A. 2010 Sep 14;107(37):16325-30. doi: 10.1073/pnas.1003459107. Epub 2010 Aug 24. Proc Natl Acad Sci U S A. 2010. PMID: 20736350 Free PMC article.
-
The relationship between SMN, the spinal muscular atrophy protein, and nuclear coiled bodies in differentiated tissues and cultured cells.Exp Cell Res. 2000 May 1;256(2):365-74. doi: 10.1006/excr.2000.4858. Exp Cell Res. 2000. PMID: 10772809
-
Association of the nuclear matrix component NuMA with the Cajal body and nuclear speckle compartments during transitions in transcriptional activity in lens cell differentiation.Eur J Cell Biol. 2002 Oct;81(10):557-66. doi: 10.1078/0171-9335-00275. Eur J Cell Biol. 2002. PMID: 12437190
-
Nuclear bodies and compartments: functional roles and cellular signalling in health and disease.Cell Signal. 2004 Oct;16(10):1085-104. doi: 10.1016/j.cellsig.2004.03.020. Cell Signal. 2004. PMID: 15240004 Review.
Cited by
-
Spinal muscular atrophy and a model for survival of motor neuron protein function in axonal ribonucleoprotein complexes.Results Probl Cell Differ. 2009;48:289-326. doi: 10.1007/400_2009_4. Results Probl Cell Differ. 2009. PMID: 19343312 Free PMC article.
-
Internal dynamics of a living cell nucleus investigated by dynamic light scattering.Eur Phys J E Soft Matter. 2008 Aug;26(4):435-48. doi: 10.1140/epje/i2007-10346-5. Eur Phys J E Soft Matter. 2008. PMID: 19230214
-
TDP-43 overexpression enhances exon 7 inclusion during the survival of motor neuron pre-mRNA splicing.J Biol Chem. 2008 Oct 24;283(43):28852-9. doi: 10.1074/jbc.M805376200. Epub 2008 Aug 14. J Biol Chem. 2008. PMID: 18703504 Free PMC article.
-
RNA-binding proteins with prion-like domains in ALS and FTLD-U.Prion. 2011 Jul-Sep;5(3):179-87. doi: 10.4161/pri.5.3.17230. Epub 2011 Jul 1. Prion. 2011. PMID: 21847013 Free PMC article. Review.
-
Assays for the identification and prioritization of drug candidates for spinal muscular atrophy.Assay Drug Dev Technol. 2014 Aug;12(6):315-41. doi: 10.1089/adt.2014.587. Assay Drug Dev Technol. 2014. PMID: 25147906 Free PMC article. Review.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials