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. 1975 May;1(5):420-4.
doi: 10.1128/jcm.1.5.420-424.1975.

Rapid conversion of Histoplasma capsulatum, Blastomyces dermatitidis and sporothrix schenckii in tissue culture

H Hempel, N L Goodman

Rapid conversion of Histoplasma capsulatum, Blastomyces dermatitidis and sporothrix schenckii in tissue culture

H Hempel et al. J Clin Microbiol. 1975 May.

Abstract

A simple method for positive identification of Histoplasma capsulatum, Blastomyces dermatitidis, and Sporothrix schenckii is given. Primary tissue cultures of guinea pig peritoneal macrophage were inoculated with the mycelial phase of each organism and after 24 h the cells were stained and observed microscopically. The characteristic yeast phase could then be observed allowing for positive identification.

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References

    1. Proc Soc Exp Biol Med. 1956 Dec;93(3):612-5 - PubMed
    1. Proc Soc Exp Biol Med. 1962 Nov;111:514-21 - PubMed

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