Elution and reconcentration of polioviruses adsorbed on coal from water samples

Abstract

Viruses adsorbed on coal particles in the form of coal bed could efficiently be recovered with elution of 6% beef extract solution in Mcllvaine buffer at pH 7.1. Significant reduction in large volume of eluate could be achieved by employing modified organic flocculation technique to obtain virus concentrate. Modified organic flocculation technique involves addition of 0.02% bovine albumin in eluate. Enteroviruses were adsorbed to flocs which were formed at pH 3.5 and were recovered by centrifugation followed by solublization of flocs in a small volume of 0.15 M disodium hydrogen phosphate solution at pH 9.5. The recovery of viruses ranged from 87.9% to 97.3% with an average of 92.2%.