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. 2002 Nov 4;12(21):3093-6.
doi: 10.1016/s0960-894x(02)00650-9.

A general synthesis of specifically deuterated nucleotides for studies of DNA and RNA

Bingzi Chen  1 , Elizabeth R Jamieson, Thomas D Tullius
Affiliations

A general synthesis of specifically deuterated nucleotides for studies of DNA and RNA

Bingzi Chen et al. Bioorg Med Chem Lett. .

Abstract

An efficient procedure is described for the preparation of ribonucleotides and deoxyribonucleotides with deuterium incorporated at the 1', 4', or 5' position. Three intermediates-[1-2H]-D-ribose, [4-2H]-D-ribose, and [5-2H(2)]-D-ribose-were prepared by chemical synthesis and subsequently converted to ribonucleotides and deoxyribonucleotides via enzymatic reactions. Milligram quantities of the desired products were obtained with an average deuterium content of 96+/-1%.

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