Calf anticular cartilage in organ culture in a chemically defined medium. I. Autoradiographic study after [35S]sulfate labeling

Abstract

Articular cartilage from 5-month-old calves survived for 4 weeks in organ culture in a chemically defined medium. The viability was assessed by calculating the labeling frequency of chondrocytes on autoradiograms after [35S]sulfate labeling. The survival rate of chondrocytes was highest in 20% oxygen tension, whereas 50% oxygen resulted in chondrocyte death after 2 weeks. The survival rate was improved by using 1-mm thick standardized pieces of cartilage. The cells of the superficial layer showed a higher proportion of living chondrocytes than the cells in the deeper layers. No differentiation, dedifferentiation, or outgrowth of cells occurred during the experimental period.