The effects of vitamin E succinate on the expression of c-jun gene and protein in human gastric cancer SGC-7901 cells

Abstract

Aim: To investigate the effects of vitamin E succinate (VES) on the expression of c-jun gene and protein in human gastric cancer SGC-7901 cells.

Methods: After SGC-7901 cells were treated with VES at different doses (5,10,20 mg x L(-1)) at different time, reverse transcription-PCR technique was used to detect the level of c-jun mRNA; Western Blot was applied to measure the expression of c-jun protein.

Results: After the cells were treated with VES at 20 mg x L(-1) for 3 h, the expression rapidly reached its maximum that was 3.5 times of UT control (P<0.01). The level of c-jun mRNA was also increased following treatment of VES for 6 h. However,the expression after treatment of VES at 5 mg x L(-1) for 24 h was 1.6 times compared with UT control (P<0.01). Western blot analysis showed that the level of c-jun protein was obviously elevated in VES-treated SGC-7901 cells at 20 mg x L(-1) for 3 h. The expression of c-jun protein was gradually increased after treatment of VES at 20 mg x L(-1) for 3, 6, 12 and 24 h, respectively, with an evident time-effect relationship.

Conclusion: The levels of c-jun mRNA and protein in VES-treated SGC-7901 cells were increased in a dose- and time-dependent manner; the expression of c-jun was prolonged by VES, indicating that c-jun is involved in VES-induced apoptosis in SGC-7901 cells.

Figure 1

Effect of VES on the expression of c-jun mRNA in SGC-7901 cells for different time points by RT-PCR. A: treatment of VES for 3 h; B: treatment of VES for 6 h; C: treatment of VES for 24 h.1: UT control; 2: succinate; 3: vitamin E; 4: VES at 5 mg•L^-1; 5: VES at 10 mg•L^-1; 6: VES at 20 mg•L^-1; M: molecular weight marker.

Figure 2

The expression of c-jun protein in SGC-7901 cells following treatment of VES for 3 h. Lane1: Molecular weight marker; Lane2: UT control; Lane3: succinate; Lane4: vitamin E; Lane5: VES at 5 mg•L^-1; Lane6: VES at 10 mg•L^-1; Lane7:VES at 20 mg•L^-1.

Figure 3

The expression of c-jun protein in SGC-7901 cells following treatment of VES at 20 mg•L^-1 for different time poits. Lane1: Molecular weight marker; Lane2: 3 h; Lane3: 6 h; Lane4: 12 h; Lane5: 24 h.