Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 Oct;8(5):782-6.
doi: 10.3748/wjg.v8.i5.782.

The effects of vitamin E succinate on the expression of c-jun gene and protein in human gastric cancer SGC-7901 cells

Yan Zhao  1 , Kun Wu, Wei Xia, Yu-Juan Shan, Li-Jie Wu, Wei-Ping Yu
Affiliations

The effects of vitamin E succinate on the expression of c-jun gene and protein in human gastric cancer SGC-7901 cells

Yan Zhao et al. World J Gastroenterol. 2002 Oct.

Abstract

Aim: To investigate the effects of vitamin E succinate (VES) on the expression of c-jun gene and protein in human gastric cancer SGC-7901 cells.

Methods: After SGC-7901 cells were treated with VES at different doses (5,10,20 mg x L(-1)) at different time, reverse transcription-PCR technique was used to detect the level of c-jun mRNA; Western Blot was applied to measure the expression of c-jun protein.

Results: After the cells were treated with VES at 20 mg x L(-1) for 3 h, the expression rapidly reached its maximum that was 3.5 times of UT control (P<0.01). The level of c-jun mRNA was also increased following treatment of VES for 6 h. However,the expression after treatment of VES at 5 mg x L(-1) for 24 h was 1.6 times compared with UT control (P<0.01). Western blot analysis showed that the level of c-jun protein was obviously elevated in VES-treated SGC-7901 cells at 20 mg x L(-1) for 3 h. The expression of c-jun protein was gradually increased after treatment of VES at 20 mg x L(-1) for 3, 6, 12 and 24 h, respectively, with an evident time-effect relationship.

Conclusion: The levels of c-jun mRNA and protein in VES-treated SGC-7901 cells were increased in a dose- and time-dependent manner; the expression of c-jun was prolonged by VES, indicating that c-jun is involved in VES-induced apoptosis in SGC-7901 cells.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Effect of VES on the expression of c-jun mRNA in SGC-7901 cells for different time points by RT-PCR. A: treatment of VES for 3 h; B: treatment of VES for 6 h; C: treatment of VES for 24 h.1: UT control; 2: succinate; 3: vitamin E; 4: VES at 5 mg•L-1; 5: VES at 10 mg•L-1; 6: VES at 20 mg•L-1; M: molecular weight marker.
Figure 2
Figure 2
The expression of c-jun protein in SGC-7901 cells following treatment of VES for 3 h. Lane1: Molecular weight marker; Lane2: UT control; Lane3: succinate; Lane4: vitamin E; Lane5: VES at 5 mg•L-1; Lane6: VES at 10 mg•L-1; Lane7:VES at 20 mg•L-1.
Figure 3
Figure 3
The expression of c-jun protein in SGC-7901 cells following treatment of VES at 20 mg•L-1 for different time poits. Lane1: Molecular weight marker; Lane2: 3 h; Lane3: 6 h; Lane4: 12 h; Lane5: 24 h.
See this image and copyright information in PMC

References

    1. Fariss MW, Fortuna MB, Everett CK, Smith JD, Trent DF, Djuric Z. The selective antiproliferative effects of alpha-tocopheryl hemisuccinate and cholesteryl hemisuccinate on murine leukemia cells result from the action of the intact compounds. Cancer Res. 1994;54:3346–3351. - PubMed
    1. Ottino P, Duncan JR. Effect of alpha-tocopherol succinate on free radical and lipid peroxidation levels in BL6 melanoma cells. Free Radic Biol Med. 1997;22:1145–1151. - PubMed
    1. Israel K, Sanders BG, Kline K. RRR-alpha-tocopheryl succinate inhibits the proliferation of human prostatic tumor cells with defective cell cycle/differentiation pathways. Nutr Cancer. 1995;24:161–169. - PubMed
    1. Turley JM, Ruscetti FW, Kim SJ, Fu T, Gou FV, Birchenall-Roberts MC. Vitamin E succinate inhibits proliferation of BT-20 human breast cancer cells: increased binding of cyclin A negatively regulates E2F transactivation activity. Cancer Res. 1997;57:2668–2675. - PubMed
    1. Neuzil J, Weber T, Gellert N, Weber C. Selective cancer cell killing by alpha-tocopheryl succinate. Br J Cancer. 2001;84:87–89. - PMC - PubMed

Publication types

MeSH terms