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. 2002 Oct;8(5):897-900.
doi: 10.3748/wjg.v8.i5.897.

Effect of N-desulfated heparin on hepatic/renal ischemia reperfusion injury in rats

Tong Zhou  1 , Jin-Lian Chen, Wei Song, Feng Wang, Ming-Jun Zhang, Pei-Hua Ni, Jian-Guo Geng
Affiliations

Effect of N-desulfated heparin on hepatic/renal ischemia reperfusion injury in rats

Tong Zhou et al. World J Gastroenterol. 2002 Oct.

Abstract

Aim: To investigate the effect of N-desulfated heparin on hepatic/renal ischemia and reperfusion injury in rats.

Methods: Using rat models of 60 minutes hepatic or renal ischemia followed by 1 h,3 h,6 h and 24 h reperfusion,animals were randomly divided into following groups,the sham operated controls,ischemic group receiving only normal saline,and treated group receiving N-desulfated heparin at a dose of 12 mg/kg at 5 minutes before reperfusion. P-selectin expression was detected in hepatic/renal tissues with immunohistochemistry method.

Results: P-selectin expression, serum ALT, AST, BUN and Cr levels were significantly increased during 60 minute ischemia and 1 h, 3 h, 6 h and 24 h reperfusion,while the increment was significantly inhibited,and hepatic/renal pathology observed by light microscopy was remarkably improved by treatment with the N-desulfated heparin. Furthermore,the heparin was found no effects on PT and KPTT.

Conclusion: P-selectin might mediate neutrophil infiltration and contribute to hepatic/renal ischemia and reperfusion. The N-desulfated heparin might prevent hepatic/renal damage induced by ischemia and reperfusion injury without significant anticoagulant activity.

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