[Catalytic properties of cytoplasmic and mitochondrial aconitate hydratase from rat cardiomyocytes]
[Article in
Russian]
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- PMID: 12400375
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[Catalytic properties of cytoplasmic and mitochondrial aconitate hydratase from rat cardiomyocytes]
[Article in
Russian]
Izv Akad Nauk Ser Biol.
2002 Sep-Oct.
Abstract
Enzymatic activity of aconitate hydratase (aconitase, EC 4.2.1.3) from the rat heart is localized in the cytoplasm (65%) and mitochondria (35%). Cytoplasmic and mitochondrial forms of aconitate hydratase were separated by ion-exchange chromatography on CM-Cellulose and CM-Sephadex. The two forms have similar molecular weight, optimal pH range, and spectral properties; however, they have different chromatography properties, Km for citrate and isocitrate, as well as sensitivity to Fe2+ ions.
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