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. 2002 Nov 1;30(21):e118.
doi: 10.1093/nar/gnf117.

BACFinder: genomic localisation of large insert genomic clones based on restriction fingerprinting

Mark L Crowe  1 , Debashis Rana, Fiona Fraser, Ian Bancroft, Martin Trick
Affiliations

BACFinder: genomic localisation of large insert genomic clones based on restriction fingerprinting

Mark L Crowe et al. Nucleic Acids Res. .

Abstract

We have developed software that allows the prediction of the genomic location of a bacterial artificial chromosome (BAC) clone, or other large genomic clone, based on a simple restriction digest of the BAC. The mapping is performed by comparing the experimentally derived restriction digest of the BAC DNA with a virtual restriction digest of the whole genome sequence. Our trials indicate that this program identified the genomic regions represented by BAC clones with a degree of accuracy comparable to that of end-sequencing, but at considerably less cost. Although the program has been developed principally for use with Arabidopsis BACs, it should align large insert genomic clones to any fully sequenced genome.

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