Agrocybe cylindracea lectin is a member of the galectin family

Abstract

The complete amino acid sequence of Agrocybe cylindracea lectin was determined from the peptides obtained by chemical cleavages and enzymatic hydrolyses. The sequence shows 19.1% and 36.8% identity with those of human galectin-1 and Coprinus lectin-1, a fungal galectin, respectively. Seven residues, which are commonly found in carbohydrate recognizing domain (CRD) of galectins, were conserved. However, several insertions in the sequence, compared with those of human galectin-1 and Coprinus lectin-1, suggest that beta-strands S2, F3, and S4 and the loop structures between beta-strands F2 & S3 and F5 & S2 are different from those of galectins reported so far.