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. 2002 Nov;2(11):1630-2.
doi: 10.1002/1615-9861(200211)2:11<1630::AID-PROT1630>3.0.CO;2-N.

Organic disulfides as a means to generate streak-free two-dimensional maps with narrow range basic immobilized pH gradient strips as first dimension

Ingmar Olsson  1 , Kjell Larsson, Ronnie Palmgren, Bengt Bjellqvist
Affiliations

Organic disulfides as a means to generate streak-free two-dimensional maps with narrow range basic immobilized pH gradient strips as first dimension

Ingmar Olsson et al. Proteomics. 2002 Nov.

Abstract

Streaking is a severe problem when narrow range basic immobilized pH gradient strips are used as the first dimension of two-dimensional (2-D) electrophoresis. It is demonstrated that this cysteinyl related streaking is eliminated when focusing is done in the presence of hydroxyethyl disulfide (DeStreak). Use of DeStreak also results in 2-D maps with simplified spot patterns and improved reproducibility.

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