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. 2002 Nov;8(11):1192-6.
doi: 10.3201/eid0811.020296.

National Tuberculosis Genotyping and Surveillance Network: design and methods

Jack T Crawford  1 , Christopher R Braden, Barbara A Schable, Ida M Onorato
Affiliations

National Tuberculosis Genotyping and Surveillance Network: design and methods

Jack T Crawford et al. Emerg Infect Dis. 2002 Nov.

Abstract

The National Tuberculosis Genotyping and Surveillance Network was established in 1996 to perform a 5-year, prospective study of the usefulness of genotyping Mycobacterium tuberculosis isolates to tuberculosis control programs. Seven sentinel sites identified all new cases of tuberculosis, collected information on patients and contacts, and obtained patient isolates. Seven genotyping laboratories performed DNA fingerprinting analysis by the international standard IS6110 method. BioImage Whole Band Analyzer software was used to analyze patterns, and distinct patterns were assigned unique designations. Isolates with six or fewer bands on IS6110 patterns were also spoligotyped. Patient data and genotyping designations were entered in a relational database and merged with selected variables from the national surveillance database. In two related databases, we compiled the results of routine contact investigations and the results of investigations of the relationships of patients who had isolates with matching genotypes. We describe the methods used in the study.

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Jack T. Crawford, Guest Editor. Dr. Crawford is chief of the Immunology and Molecular Pathogenesis Section, Tuberculosis/Mycobacteriology Branch, Division of AIDS, STD, and TB Laboratory Research, Centers for Disease Control and Prevention. His research interests include application of molecular methods to epidemiology and diagnostics of mycobacterial diseases.
Figure
Figure
Map showing locations of the sentinel surveillance sites in the National Tuberculosis Genotyping and Surveillance Network, United States.
See this image and copyright information in PMC

References

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