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. 2002 Dec;40(12):4776-8.
doi: 10.1128/JCM.40.12.4776-4778.2002.

Loss of a 29-kilodalton outer membrane protein in Acinetobacter baumannii is associated with imipenem resistance

Adriana S Limansky  1 , María Alejandra Mussi, Alejandro M Viale
Affiliations

Loss of a 29-kilodalton outer membrane protein in Acinetobacter baumannii is associated with imipenem resistance

Adriana S Limansky et al. J Clin Microbiol. 2002 Dec.

Abstract

We analyzed the possible causes of imipenem (IPM) resistance in multidrug-resistant isolates of Acinetobacter baumannii. Comparison of the outer membrane protein (OMP) profiles of two genomically related strains (Ab288 [IPM sensitive] and Ab242 [IPM resistant]) indicated the conspicuous loss of a 29-kDa polypeptide in the Ab242 strain. No carbapenemase activity was detected in any of these strains. The treatment of Ab288 with sodium salicylate resulted in IPM resistance and the loss of the 29-kDa OMP. In addition, IPM-resistant clones of Ab288 which were selected by repetitive culturing in increasing concentrations of this antibiotic also showed the absence of this 29-kDa OMP.

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Figures

FIG. 1.
FIG. 1.
IPM resistance is linked to the loss of a 29-kDa OMP in A. baumannii. The corresponding purified outer membrane fractions (∼30 μg of protein) were incubated with 2% SDS for 5 min in a boiling water bath, subjected to SDS-PAGE as described in the text, and stained with Coomassie blue. (A) OMP profiles of IPM-sensitive (Ab288) and IPM-resistant (Ab242) clinical strains. (B) OMP profiles of Ab288, salicylate-treated Ab288 (Ab288s), and an IPM-resistant mutant clone of Ab288 (Ab288m). The closed arrowheads at the right margins indicate the position of the 29-kDa OMP, and the locations and molecular masses of the protein markers are indicated at the left margins. Corel DRAW 9.0 software was used.
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