Distribution of cholesterol-5alpha,6alpha-epoxide formation and its metabolism in mouse skin

Abstract

The distribution of cholesterol-5alpha,6alpha-epoxide in ultraviolet light-irradiated mouse skin was determined. Highest levels of cholesterol-5alpha,6alpha-epoxide were found in the epidermis and distributed rather evenly throughout all subcellular fractions. Low but potentially significant levels of the compound diffused from the epidermis. Metabolic studies demonstrated that cholesterol-5alpha,6alpha-epoxide was converted to cholestane-3ss,5alpha,6ss-triol by an enzyme, cholesterol-5alpha,6alpha-epoxide hydrase, which was localized in the epidermal fractions sedimenting at 10,000 and 100,000 X g. Comparative studies indicated a much higher capacity for metabolism of cholesterol-5alpha,6alpha-expoide in liver than in skin. The effect of the epoxide and its metabolic product on the skin is discussed.