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. 1976 Nov 30;13(2):95-100.
doi: 10.1007/BF01837059.

The extracellular metalloprotease of Serratia marcescens: I. Purification and characterization

P S Aiyappa, J O Harris

The extracellular metalloprotease of Serratia marcescens: I. Purification and characterization

P S Aiyappa et al. Mol Cell Biochem. .

Abstract

An extracellular protease of Serratia marcescens produced during growth on skim milk medium was isolated by ethanol precipitation. The protease was purified by salt fractionation, DEAE-cellulose ion exchange chromatography and gel filtration chromatography on Agarose P-100. It has a broad optimum from pH 6.0 to 9.0 and a temperature optimum of 45 degrees C for proteolytic activity on casein. It was classified as a metallo-protease by virtue of its inactivation by metal-ion chelators and reactivation by ferrous ions. Proteolytic activity was not affected by diiso-propylfluorophosphate, p-chloromercuribenzoate and dithiothreitol.

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References

    1. Annu Rev Biochem. 1960;29:45-72 - PubMed
    1. Ann N Y Acad Sci. 1964 Dec 28;121:404-27 - PubMed
    1. Folia Microbiol (Praha). 1970;15(3):160-8 - PubMed
    1. J Biol Chem. 1952 Feb;194(2):721-7 - PubMed
    1. Biochem J. 1965 Sep;96(3):595-606 - PubMed