doi: 10.1126/science.1246629.
Measurement of membrane protein lateral diffusion in single cells
- PMID: 1246629
- DOI: 10.1126/science.1246629
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Measurement of membrane protein lateral diffusion in single cells
Science.
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Abstract
Fluorescence rapidly returns to spots bleached by a laser beam in the continuous fluorescence of cultured cells labeled on the surface with fluorescein isothiocyanate. The rate of recovery of fluorescence after bleaching can be interpreted as a measure of the lateral diffusion of integral membrane proteins labeled with fluorescein.
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