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Comparative Study
. 2002 Nov;32(6):445-57.
doi: 10.1023/a:1020832327983.

Voluntary consumption of NaCl, KCl, CaCl2, and NH4Cl solutions by 28 mouse strains

Alexander A Bachmanov  1 , Gary K Beauchamp, Michael G Tordoff
Affiliations
Comparative Study

Voluntary consumption of NaCl, KCl, CaCl2, and NH4Cl solutions by 28 mouse strains

Alexander A Bachmanov et al. Behav Genet. 2002 Nov.

Abstract

Male mice from 28 inbred strains (129P3/J, A/J, AKR/J, BALB/cByJ, BUB/BnJ, C3H/HeJ, C57BL/6J, C57L/J, CAST/Ei, CBA/J, CE/J, DBA/2J, FVB/NJ, I/LnJ, KK/H1J, LP/J, NOD/LtJ, NZB/B1NJ, P/J, PL/J, RBF/DnJ, RF/J, RIIIS/J, SEA/GnJ, SJL/J, SM/J, SPRET/Ei, and SWR/J) were tested with NaCl (75-450 mM), KCl (30-300 mM), CaCl2 (3-100 mM), and NH4Cl (10-300 mM) solutions using two-bottle preference tests with water as the second choice. For each mineral, there was a wide range of strain variation in solution intakes and preferences. This variation had a substantial genetic component as assessed using heritability estimates. In most cases, the strain means were continuously distributed; however, strains with deviating high or low intakes or preferences were also observed. The associations among the responses to different minerals were only modest, suggesting distinct genetic controls of sodium, potassium, calcium, and ammonium consumption. These results provide a valuable resource for investigators who wish to identify genes involved in the regulation of mineral consumption and balance.

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Figures

Fig. 1
Fig. 1
NaCl concentration-intake (top panels) and concentration-preference (bottom panels) functions for 28 strains of mice. Differences between strains larger than the Tukey’s critical difference (CD) given in Table II are significant.
Fig. 2
Fig. 2
KCl concentration-intake (top panels) and concentration-preference (bottom panels) functions for 28 strains of mice. Differences between strains larger than the Tukey’s CD given in Table II are significant.
Fig. 3
Fig. 3
CaCl2 concentration-intake (top panels) and concentration-preference (bottom panels) functions for 28 strains of mice. Differences between strains larger than the Tukey’s CD given in Table II are significant.
Fig. 4
Fig. 4
NH4Cl concentration-intake (top panels) and concentration-preference (bottom panels) functions for 28 strains of mice. Differences between strains larger than the Tukey’s CD given in Table II are significant.
Fig. 5
Fig. 5
Strain distribution of intakes and preferences for each concentration of four taste solutions. Where possible, outlying points have been labeled with an abbreviated strain name. Differences between strains larger than the Tukey’s CD given in Table II are significant. Note that the scale for NaCl intake (top LH panel) is twice that of the other three mineral salt intakes.
Fig. 6
Fig. 6
Heritability estimates for intakes and preferences for each concentration of four taste solutions. Heritability was calculated as a ratio SSamong strains/SStotal based on the sums of squares (SS) obtained in one-way ANOVAs for each concentration (Bachmanov et al., 2002).
Fig. 7
Fig. 7
Tree diagram for salt solution preferences obtained using cluster analysis.
See this image and copyright information in PMC

References

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    1. Bachmanov AA, Inoue M, Ninomiya Y, Beauchamp GK. Modification of behavioral and neural taste responses to NaCl in C57BL/6 mice: Effects of NaCl exposure and DOCA treatment. Physiol Behav. 1999;65:817–822. - PMC - PubMed
    1. Bachmanov AA, Reed DR, Beauchamp GK, Tordoff MG. Food intake, water intake, and drinking spout side preference of 28 mouse strains. Behav Genet. 2002;32:435–443. - PMC - PubMed

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