Pre-proparathyroid hormone: analysis of radioactive tryptic peptides and amino acid sequence
- PMID: 1247503
- DOI: 10.1021/bi00646a003
Pre-proparathyroid hormone: analysis of radioactive tryptic peptides and amino acid sequence
Abstract
The amino acid sequence of bovine pre-proparathyroid hormone has been partially determined by analysis of the polypeptide labeled selectively with radioactive amino acids. Analysis of tryptic peptides containing methionine or lysine indicated that parathyroid hormone, proparathyroid hormone, and pre-proparathyroid hormone had several common peptides. Two lysine-containing peptides present in proparathyroid hormone but not in parathyroid hormone were also present in pre-proparathyroid hormone. In addition, pre-proparathyroid hormone contained several additional lysine- and methionine-containing peptides not present in parathyroid hormone or proparathyroid hormone. Analysis by repetitive Edman degradation of the polypeptide labeled with lysine, methionine, and other amino acids indicated that pre-proparathyroid hormone contained 25 additional amino acids at the amino terminus of proparathyroid hormone; the identities of 17 of the 25 amino acids have been established. An unusual feature found was the presence of methionyl-methionyl at the amino terminus and the presence of 5 methionines within the first 14 amino acids.
Similar articles
-
Pre-proparathyroid hormone: fidelity of the translation of parathyroid messenger RNA by extracts of wheat germ.Biochemistry. 1976 Jan 13;15(1):20-5. doi: 10.1021/bi00646a004. Biochemistry. 1976. PMID: 1247507
-
Pre-proparathyroid hormone: a direct translation product of parathyroid messenger RNA.Proc Natl Acad Sci U S A. 1974 Sep;71(9):3731-5. doi: 10.1073/pnas.71.9.3731. Proc Natl Acad Sci U S A. 1974. PMID: 4530333 Free PMC article.
-
Pre-proparathyroid hormone. Evidence for an early biosynthetic precursor of proparathyroid hormone.J Biol Chem. 1976 Jul 10;251(13):3893-9. J Biol Chem. 1976. PMID: 932012
-
biosynthesis of pre-proparathyroid hormone.Methods Cell Biol. 1981;23:51-71. doi: 10.1016/s0091-679x(08)61491-0. Methods Cell Biol. 1981. PMID: 7035810 Review. No abstract available.
-
Parathyroid hormone: chemistry and structure-activity relations.Pathobiol Annu. 1981;11:53-86. Pathobiol Annu. 1981. PMID: 6276847 Review.
Cited by
-
Purification and translation of zein messenger RNA from maize endosperm protein bodies.Proc Natl Acad Sci U S A. 1978 Feb;75(2):696-700. doi: 10.1073/pnas.75.2.696. Proc Natl Acad Sci U S A. 1978. PMID: 16592496 Free PMC article.
-
Partial amino acid sequence of rat pre-prolactin.Biochem J. 1977 Jan 1;161(1):189-92. doi: 10.1042/bj1610189. Biochem J. 1977. PMID: 851420 Free PMC article.
-
Amino acid sequence of the NH2-terminal extra piece segments of the precursors of mouse immunoglobulin lambda1-type and kappa-type light chains.Proc Natl Acad Sci U S A. 1977 Feb;74(2):716-20. doi: 10.1073/pnas.74.2.716. Proc Natl Acad Sci U S A. 1977. PMID: 403522 Free PMC article.
-
Studies on the intracellular segregation of polyribosome-associated messenger ribonucleic acid species in the lactating guinea-pig mammary gland.Biochem J. 1979 Sep 1;181(3):737-56. doi: 10.1042/bj1810737. Biochem J. 1979. PMID: 518553 Free PMC article.
-
Characterization of an endopeptidase involved in pre-protein processing.Proc Natl Acad Sci U S A. 1979 Sep;76(9):4225-9. doi: 10.1073/pnas.76.9.4225. Proc Natl Acad Sci U S A. 1979. PMID: 291960 Free PMC article.