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. 2002 Nov;19(11):536-8.
doi: 10.1023/a:1020916003666.

Tissue sampling technique affects accuracy of karyotype from missed abortions

Ruth B Lathi  1 , Amin A Milki
Affiliations

Tissue sampling technique affects accuracy of karyotype from missed abortions

Ruth B Lathi et al. J Assist Reprod Genet. 2002 Nov.

Abstract

Purpose: To determine if careful specimen selection and washing of tissue from first trimester missed abortion products of conception specimens increases the sensitivity of routine cytogenetics in detecting aneuploidy.

Methods: Retrospective review of cytogenetics results from tissue from dilation and curettage for missed abortion in a university fertility practice between 1998 and 2001. A technique of careful selection and washing of the specimen was implemented in July 1999. Results from before (n = 15) and after (n = 41) this change were compared. Cytogenetics reports from other physicians using the same laboratory were used for comparison (n = 59).

Results: The percentage of 46XX results was significantly decreased in the test group when compared to historical and community controls: 29% vs. 73% and 56% respectively. The percentage of aneuploid results was significantly higher in the test group at 61% vs. 7% and 36% in the historical and community controls respectively.

Conclusion: Thorough separation and cleaning of villi prior to sending missed abortion specimens significantly increases sensitivity of conventional cytogenetics for detecting aneuploidy by decreasing maternal contamination.

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