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Comment
. 2002 Dec 24;99(26):16516-8.
doi: 10.1073/pnas.012694899. Epub 2002 Dec 16.

Design then mutate

Jeff Hasty  1
Affiliations
Comment

Design then mutate

Jeff Hasty. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Fig 1.
Fig 1.
The essential interactions of the three-gene regulatory circuit explored by Yokobayashi et al. (1).
Fig 2.
Fig 2.
The coupling of mutagenesis with epigenetic type behavior. (Upper) A generic two-gene network consisting of the genes x and y. Both proteins (X and Y) are under the control of a promoter that is activated by the protein X, and protein Y is a protease for X. Oscillations can arise because Y degrades X and thus reduces its own expression level (because X activates transcription of y). (Lower) Depending on the parameter values, the network can exhibit different phenotypic behaviors. A phase plane diagram indicates the degradation rates for X and Y that lead to either equilibrium or oscillatory behavior for the network. The path from point 1 to point 2 depicts how mutations that increase the degradation rate of Y could result in an abrupt change in the output behavior of X (and thus Y) from equilibrium to oscillatory behavior. See ref. for a detailed analysis of this model network and ref. for an experimental demonstration of oscillations in a synthetic gene network.
See this image and copyright information in PMC

Comment on

  • Directed evolution of a genetic circuit.
    Yokobayashi Y, Weiss R, Arnold FH. Yokobayashi Y, et al. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16587-91. doi: 10.1073/pnas.252535999. Epub 2002 Nov 25. Proc Natl Acad Sci U S A. 2002. PMID: 12451174 Free PMC article.

References

    1. Yokobayashi Y., Weiss, R. & Arnold, F. H. (2002) Proc. Natl. Acad. Sci. USA 99, 16587-16591. - PMC - PubMed
    1. Farinas E., Tulter, T. & Arnold, F. H. (2001) Curr. Opin. Biotechnol. 6, 545-551. - PubMed
    1. Weiss R., (2001) Ph.D. thesis (Massachusetts Institute of Technology, Cambridge).
    1. Csete M. E. & Doyle, J. C. (2002) Science 295, 1664-1669. - PubMed
    1. Ideker T., Thorsson, V., Ranish, J. A., Christmas, R., Buhler, J., Eng, J. K., Bumgarner, R., Goodlet, D. R., Aebersold, R. & Hood, L. (2001) Science 292, 929-934. - PubMed

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