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. 1976 Feb 18;117(2):399-405.
doi: 10.1016/0021-9673(76)80017-9.

The determination of gamma-amino-beta-(p-chlorophenyl)butyric acid (baclofen) in biological material by gas-liquid chromatography

P H Degen, W Riess

The determination of gamma-amino-beta-(p-chlorophenyl)butyric acid (baclofen) in biological material by gas-liquid chromatography

P H Degen et al. J Chromatogr. .

Abstract

Baclofen and the internal standard are isolated from biological samples by adsorption on charcoal. After elution, the compounds are converted into butyl esters and purified by base-specific solvent extraction. The butyl esters are then N-acylated with heptafluorobutyrylimidazole, and the neutral derivatives are isolated by solvent extraction and subjected to gas-liquid chromarography, with use of an electron-capture detector. Baclofen can be determined in concentrations down to about 30 ng per sample.

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