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. 1975 Jun 27;188(4195):1308-10.
doi: 10.1126/science.124948.

HL-A LD (lymphocyte defined) typing: a rapid assay with primed lymphocytes

M J Sheehy, P M Sondel, M L Bach, R Wank, F H Bach

HL-A LD (lymphocyte defined) typing: a rapid assay with primed lymphocytes

M J Sheehy et al. Science. .

Abstract

When human lymphocytes are cultured for 9 to 14 days with stimulating cells of a family member differing by a single HL-A haplotype they become "primed" to recognize specific HL-A LD (mixed lymphocyte culture) antigens. These primed lymphocytes respond specifically and rapidly when "restimulated" with cells of a person that contain the same LD antigens as those of the priming haplotype. Specific HL-A LD antigens can be detected within 24 hours by this primed LD typing.

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