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Comparative Study
. 2002;10(7):571-7.
doi: 10.1023/a:1020914702767.

The use of laser microdissection for the preparation of chromosome-specific painting probes in farm animals

Svatava Kubickova  1 , Halina Cernohorska, Petra Musilova, Jiri Rubes
Affiliations
Comparative Study

The use of laser microdissection for the preparation of chromosome-specific painting probes in farm animals

Svatava Kubickova et al. Chromosome Res. 2002.

Abstract

Laser microbeam microdissection and laser pressure catapulting procedure were used for the construction of chromosome-specific painting probes, arm-specific probes and probes for chromosomal subfragments. We report on a method for generation of fluorescence in-situ hybridization probes from laser dissected chromosomes of farm animals. So far, using the described method, a set of chromosome-specific painting probes has been obtained for all porcine chromosomes, 17 chromosomes of cattle and selected equine chromosomes. It is concluded that the laser technology appears to be a useful and powerful tool for the construction of chromosome-specifi c painting probes. Its main advantage is the fast non-contact collection of chromosomes.

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