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. 2003 Jan;47(1):387-9.
doi: 10.1128/AAC.47.1.387-389.2003.

Site-specific mutagenesis analysis of PBP 1A from a penicillin-cephalosporin-resistant pneumococcal isolate

Anthony M Smith  1 , Keith P Klugman
Affiliations

Site-specific mutagenesis analysis of PBP 1A from a penicillin-cephalosporin-resistant pneumococcal isolate

Anthony M Smith et al. Antimicrob Agents Chemother. 2003 Jan.

Abstract

Our mutagenesis study has investigated all amino acid mutations in the penicillin-binding domain of PBP 1A from Hungarian pneumococcal isolate 3191 to determine the importance of every mutation in the development of penicillin and cefotaxime resistance. Our data reveal that mutations at amino acid positions 574 to 577 and position 539 cause penicillin and cefotaxime resistance.

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Figures

FIG. 1.
FIG. 1.
Amino acid sequences of the PBD of PBP 1A from susceptible strain R6 (line 1) and isolate 3191 (line 2). Only those positions that differ from strain R6 are shown in line 2. The conserved amino acid sequence motifs (STMK, SRN, KTG) are underlined. Sequences are numbered according to the published sequence of Martin and coworkers (7).
See this image and copyright information in PMC

References

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